CBSE Class 12 Bio-Technology Latest Syllabus 2022-2023 (Latest)
Objectives Of Bio-Technology
The broad objectives of teaching Biotechnology at the senior secondary level are to:
- help the learners know and understand basic facts and concepts of the subject in elementary
- expose the students to different basic processes and basic techniques used in
- familiarize the learners to understand the relationship of the subject to health, nutrition, environment, agriculture, and industry, etc.
- develop conceptual competence in the learners so as to cope up with professional courses in future
- acquaint students with different applications of Biotechnology in everyday
- develop an interest in students to study Biotechnology as a discipline
CLASS- XI (2022-23) COURSE STRUCTURE
One Paper Time: 3 hrs.
Max. Marks 70+30
| Units | Marks | |
| Unit- I | Biotechnology: An overview | 5 |
| Unit-II | Molecules of Life | 20 |
| Unit-III | Genetics and Molecular Biology | 20 |
| Unit-IV | Cells and Organisms | 25 |
| Practical | 30 | |
| Total | 100 |
CLASS XI
(Theory)
One Paper Time: 3 hrs.
Total Marks: 70
Unit-I Biotechnology: An overview 5 Marks
Chapter 1: Biotechnology: An Overview
Historical Perspectives, Technology, and Applications of Biotechnology, Global market and Biotech Products.
Unit-II Molecules of Life 20 Marks
Chapter 1: Biomolecules: Building Blocks
Building Blocks of Carbohydrates – Sugars and their Derivatives, Building Blocks of Proteins – Amino Acids, Building Blocks of Lipids – Simple Fatty Acids, Glycerol and Cholesterol, Building Blocks of Nucleic Acids – Nucleotides.
Chapter 2: Macromolecules: Structure & Function
Carbohydrates – The Energy Givers, Proteins – The Performers, Enzymes – The Catalysts, Lipids and Biomembranes – The Barriers, Nucleic Acids – The Managers
Unit-III Genetics and Molecular Biology 20 Marks Chapter 1: Concepts of Genetics
Historical Perspective, Multiple Alleles, Linkage, and Crossing Over, Genetic Mapping.
Chapter 2: Genes and Genomes: Structure and Function
Discovery of DNA as Genetic Material, DNA Replication, Fine Structure of the Genes, From Gene to Protein, Transcription – The Basic Process, Genetic Code, Translation, Mutations, Human Genetic Disorders.
Unit IV: Cells and Organisms 25 Marks
Chapter 1: The Basic Unit of Life
Cell Structure and Components, Organization of Life
Chapter 2: Cell Growth and Development
Cell Division, Cell Cycle, Cell Communication, Nutrition, Reproduction, Immune Response in Animals.
PRACTICALS
Note: Every student is required to do the following experiments during the academic session.
- Preparation of buffers and pH determination
- Sterilization techniques
- Preparation of bacterial growth medium
- Cell counting
- Sugar Estimation using Di Nitro Salicylic Acid test (DNS test)
- Assay for amylase enzyme
- Protein estimation by biuret method
Scheme of Evaluation
Time: 3 Hours Max. Marks 30
The scheme of evaluation at the end of the session will be as under:
Two experiments: 20
Marks Viva on experiments: 5
Marks Practical record: 5 Mark
CLASS XII (2022-23)
COURSE- STRUCTURE- (THEORY)
One Paper Max. Marks 70+30
Time: 3 hrs.
| Units | Marks | |
| Unit V | Protein and Gene Manipulation | 40 |
| Unit VI | Cell Culture and Genetic Manipulation | 30 |
| Practicals | 30 | |
| Total | 100 |
One paper Time: 3 hrs.
Total Marks: 70
Unit-V Protein and Gene Manipulation 40 Marks Chapter-1: Recombinant DNA Technology
Introduction, Tool of Recombinant DNA technology, Making rDNA molecule, Introduction of
recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), DNA Sequencing.
Chapter-2: Protein Structure and Engineering
Introduction to the world of proteins, Structure-function Relationship in proteins, Characterization of proteins, Protein based products, Designing proteins (Protein Engineering)
Chapter-3: Genomics, Proteomics and Bioinformatics
Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, Information sources, Analysis using bioinformatics tools.
Unit-VI Cell Culture and Genetic Manipulation 30 Marks
Chapter-1: Microbial Cell Culture and its Applications
Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology.
Chapter -2: Plant Cell Culture and Applications
Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Transgenic plants with beneficial traits, Biosafety of transgenic plants
Chapter-3: Animal Cell Culture and Applications
Introduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell technology.
PRACTICALS 30 Marks
Note: Every student will be required to do the following experiments during the academic session.
- Use of special equipment in biotechnology experiments
- Isolation of bacterial plasmid DNA
- Detection of DNA by gel electrophoresis
- Estimation of DNA by UV spectroscopy
- Isolation of bacteria from curd & staining of bacteria
- Cell viability assay using Evan’s blue dye exclusion method
- Data retrieval and database search using internet site NCBI and download a DNAand protein sequence from internet, analyze it and comment on it
- Reading of a DNA sequencing gel to arrive at the sequence
- Project work
Scheme of Evaluation
Time: 3 Hours Max. Marks 30
The scheme of evaluation at the end of the session will be as under:
| A | Two experiments | 6+6 (only one computer
based practical) |
| Practical record | 04 | |
| Viva on Practical | 04 | |
| B | Project work | |
| Write up | 05 | |
| Viva on project | 05 | |
| Total | 30 |
Note:- More emphasis should be given on hands on work in projects.
Prescribed Books:
- A Text Book of Biotechnology – Class XI : Published by CBSE, New Delhi
- As reference- Biotechnology – Class XI : Published by NCERT, New Delhi
- A Laboratory Manual of Biotechnology – Class XI : Published by CBSE, New Delhi
- A Text Book of Biotechnology – Class XII : Published by CBSE, New Delhi
- A Laboratory Manual of Biotechnology – Class XII : Published by CBSE, New Delhi
Assessment Areas (Theory) 2022-23 Class XII
Biotechnology (045)
Time : 3 hrs. Maximum Marks: 70 Marks
| Competencies | |
|
Demonstrate ,Knowledge and Understanding |
50% |
| Application of Knowledge / Concepts | 30% |
|
Analyse, Evaluate and Create |
20% |
Note:
- Typology of questions: VSA including MCQs, Assertion – Reasoning type questions; SA; LA-I; LA-II; Source-based/ Case-based/ Passage-based/ Integrated assessment
- An internal choice of approximately 33% would be
Suggestive verbs for various competencies
Demonstrate, Knowledge and Understanding
State, name, list, identify, define, suggest, describe, outline, summarize, etc.
Application of Knowledge/Concepts
Calculate, illustrate, show, adapt, explain, distinguish, etc.
Analyze, Evaluate and Create
Interpret, analyse, compare, contrast, examine, evaluate, discuss, construct, etc.
